Composite

Part:BBa_K1157021

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-16)

pRhl-RhlR-GFP-Pc-RhlR

This is a biosensor with positive-feedback design, for sensing C-4 AHL molecules(a type of quorum sensing(QS) molecule) produced by Psuedomonas aeruginosa. We use a constitutive promoter to express Rhl receptor, and when C-4 AHL binds with Rhl receptor(RhlR), it will bind on its specific promoter sequence pRhl, and regulate the expression of RhlR and GFP. By sensing the GFP signal, we are able to detect QS signals.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1712
    Illegal NheI site found at 1735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 322
    Illegal BamHI site found at 2009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 797
    Illegal BsaI site found at 2484
    Illegal BsaI.rc site found at 1484


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Categories
Parameters
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